A 10% homogenate (wyv) of different tissueswere prepared in a homogenizing buffer containing100 mM Tris–HCl buffer (pH 7.5), 50 mM KCl,1 mM ethylenedinitro tetra acetic acid (EDTA) and 1 mM dithiothreitol (DTT), using a motordriven Potter–Elvehjem glass homogenizer, with a Teflon pestle. The homogenate was treated with 0.5% Triton X-100 in 1:1 ratio for 30 min