Backgroun d
Dengue virus (D EN V ) ha s four differ ent ser otypes and
any one of relate d types wil l cause dengue infe ction with
the main transmis sion of mosquitoe s [ 1]. DEN V inf e c-tion with one serotyp e may drive the de velopmen t of
dengue hemor rha gic fe ver (DHF ) or dengue shock syn-drome (DSS) after re-infe ction with ano ther serotype,
which ha s a high mor tality rate (1– 2.5%) [2]. To pre vent
re-infe ction , it is imp ortant to dete ct the primary DEN V
serotype infection in travelers returning from highly en-demic areas. The laboratory DEN V diagnoses include viral
isolation, viral antigen detection, antibody detection and
genomic test. The combination of serological tests and
RT-qPCR is an approach that is often to confirm DEN V
infection [3]. L aboratory diagnostic detections in health
examination for international travelers usually need pa-tients’ serum as detecting samples. However, collection of
serum sample is time- and labor-consuming , presents a
high injury risk for infants and children, and depends on
patients’ compliance. Several studies have reported that
DEN V genome detection in urine is an alternative non-invasive diagnostic method, and the timeframes for posi-tive detection in urine are longer than they are in serum
[4-6]. Considering the severe DEN V secondary infection,
the DEN V long-last timeframe in urine sample provides a
monitoring chance rather than the serological test to de-tect positive DEN V infection with the identification of
Backgroun dDengue virus (D EN V ) ha s four differ ent ser otypes andany one of relate d types wil l cause dengue infe ction withthe main transmis sion of mosquitoe s [ 1]. DEN V inf e c-tion with one serotyp e may drive the de velopmen t ofdengue hemor rha gic fe ver (DHF ) or dengue shock syn-drome (DSS) after re-infe ction with ano ther serotype,which ha s a high mor tality rate (1– 2.5%) [2]. To pre ventre-infe ction , it is imp ortant to dete ct the primary DEN Vserotype infection in travelers returning from highly en-demic areas. The laboratory DEN V diagnoses include viralisolation, viral antigen detection, antibody detection andgenomic test. The combination of serological tests andRT-qPCR is an approach that is often to confirm DEN Vinfection [3]. L aboratory diagnostic detections in healthexamination for international travelers usually need pa-tients’ serum as detecting samples. However, collection ofserum sample is time- and labor-consuming , presents ahigh injury risk for infants and children, and depends onpatients’ compliance. Several studies have reported thatDEN V genome detection in urine is an alternative non-invasive diagnostic method, and the timeframes for posi-tive detection in urine are longer than they are in serum[4-6]. Considering the severe DEN V secondary infection,the DEN V long-last timeframe in urine sample provides amonitoring chance rather than the serological test to de-tect positive DEN V infection with the identification of
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