Free thiol group estimation
Free SH content of fish actomyosin was determined by the method of Beveridge et al. [20] using Ellman’s reagent. Samples were incubated for 15 min at 25 8C with 1 mMof Ellman’s reagent in respective buffers. Absorbance was recorded at 412 nm with appropriate blanks. The total number of thiol groups were calculated using a molar extinction coefficient of 13,500 m1 cm1 at 412 nm for thiobenzoate, released by the reaction of Ellman’s reagent from the sulfhydryl groups.