The basic culture medium consisted of-MEM (pH
7.2–7.4) supplemented with ITS (10 g/mL insulin, 5.5 g/mL transferrin,
and 5 ng/mL selenium), 2 mM glutamine, 2 mM hypoxantine, antibiotics
100 IU/mL penicillin and 100 mg/mL streptomycin, 50 g/mL ascorbic
acid, 3.0 mg/mL of bovine serum albumin (-MEM+ ).
The basic culture medium consisted of-MEM (pH7.2–7.4) supplemented with ITS (10 g/mL insulin, 5.5 g/mL transferrin,and 5 ng/mL selenium), 2 mM glutamine, 2 mM hypoxantine, antibiotics100 IU/mL penicillin and 100 mg/mL streptomycin, 50 g/mL ascorbicacid, 3.0 mg/mL of bovine serum albumin (-MEM+ ).
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