2.5. Plant regeneration from embryos
Embryos (globular, heart-shaped and cotyledonary) were transferred on semi-solid MT medium supplemented with 3.0 mM GA3 and 3% sucrose for further development of embryos (Fig. 1f). For shoot elongation plants were transferred to semi-solid MT medium supplemented with 2.68 mM NAA and 3% sucrose (Fig. 1g and h) as described previously by Koc et al. (1992). Two months later, whole plants from all examined citrus cultivars were transferred to soil mixture (1:1; torf:perlite).