Briefly, 1ml of 10mM CuCl2, 7.5mMneocuproine and 1 M NH4Ac buffer pH 7.0 solutions were added to a test tube, followed by the addition of 0.1 ml of the extract and then the total volume was adjusted to 4.1 ml with distilled water.
Briefly,1ml of 10mM CuCl2, 7.5mMneocuproine and 1 M NH4Ac bufferpH 7.0 solutions were added to a test tube, followed by theaddition of 0.1 ml of the extract and then the total volumewas adjusted to 4.1 ml with distilled water.