Additional blueberry samples were collected at the sampling locations at each of the fields in 2012 to test fruit for the presence of larvae at the time of collection. The amount of fruit collected was the same as that collected for adult rearing samples, and each sample was placed in a 3.8 L Ziplock bag with enough salt water to cover the berries. Salt water was made by mixing 236.6 ml (1 cup) of table salt (Cargill brand Top-Flo granulated salt, Cargill Salt, Minneapolis, MN) into 3.79 L of tap water. Berries were lightly crushed (just enough to break the berry skin) and samples were left
for a minimum of 1 h before placing the bag against a dark background and counting the total number of drosophila larvae floating in the liquid.