During acclimation the broodstock were fed alternately with squid Illex argentinus, blue crab Callinectes sapidus, fish Macrodon ancylodon and the commer-
cial diet (Breed S Inve Aquaculture, Belgium) four times daily. The seawater (27 °C±1) was renewed daily at a rate of 100%, and the ar-
tificial photoperiod was 14:10 light:dark.