The column was washed with water and eluted with 45% aqueous acetonitrile containing 0.1% TFA .
The active fraction was diluted with an equal volume of water and then loaded into a Daisogel SP-120-ODSB column packed with water. The column was washed with waterand eluted with 42% aqueous acetonitrile containing 0.1% TFA.
The active fraction) was diluted with an equal volume of water and then loaded into a Daisogel SP-120-ODS-B column packed with water. The column was washed with water and eluted with 40% aqueous acetonitrile containing 0.1% TFA.