Nineteen bacterial strains were evaluated for in vitro nematicidal
activity in a single experiment. A suspension containing 100 J2
nematodes in 1 mL sterilized water was applied to each 9-cm petri
dish. Then, 1 mL of whole broth culture or cell-free culture fluid of a
bacterial strain was added to each of three petri dishes containing
nematodes. The controls were sterile nutrient broth and sterile
water added to the petri dishes containing nematodes. The number
of dead nematodes, i.e., those exhibiting no movement, was
recorded after 24 h.