Because of the timeline of our four mate preference experiments
involving red males and normal males, different generations of the
source populations were used for each experiment. In some
experiments, we tested the adults collected; in other experiments,
we used offspring of collected adults. All fish collected were housed
in 39-litre aquaria and maintained in a 15:9 h light:dark room with
a recirculating water system maintained at 27 C. All groups, except
the green-diet group, were reared on an all-red food diet: AP200
when fry, live Artemia and Brine Shrimp Flake Food (Aquatic
Ecosystems, Inc., Apopka, FL, U.S.A.) when juveniles or adults.