2.1.1. Chemicals
High performance liquid chromatography (HPLC)-grade solvents
were from Acros Organics (Geel, Belgium), other chemicals
from Fluka (Buchs, Switzerland). Ultrapure water, 18 M X cm1,
0014-5793/$36.00 2010 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies