2.3. Identification of LAB. The LAB strain showing antimicrobial
activity against A. hydrophila was identified by Gram
staining,morphological observation under lightmicroscope,
standard biochemical tests, and 16S rDNA sequence analysis.
The 16S rDNA sequence analysis was performed as
follows. Bacterial genomic DNA used as template DNA
for PCR amplification of 16S rDNA was extracted from
the bacterium by using the Genomic DNA Extraction Kit
(Real Biotech Corporation, Taipei, Taiwan) according to
themanufacturer’s recommendations.The PCR amplification
of 16S rDNA was performed using two universal primers,
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