Both wild type and daf-2 lipid
droplets have higher PC to PE ratio than whole worm extracts, with a
PC/PE ratio of 3.8 (±0.6) in wild type lipid droplets compared to a 1.3
(±0.1) ratio inwholewormextracts (Fig. 1C). These ratioswere similar
in daf-2 mutant worm and lipid droplet extracts (Fig. 1C). Overall, the
lipid composition in C. elegans lipid droplets is similar to that described
in other systems, in which lipid droplets are enriched in TAGs and the
surrounding phospholipid monolayer is enriched in PC [5,35].
In addition to TAGs, the hydrophobic core of lipid droplets isolated