injection treatment did not affect broiler hatchability.However, chick quality characteristics were affected with a 12 h difference in embryo IAN. Further, the accuracy of injection into the amnion was lower in embryos belonging to the 19.0 IAN group. Histological analysis of embryos at 24 to 36 h post-in ovo injection of the EM1 vaccine revealed oral uptake of the vaccine through the amnion with the presence of coccidia life cycle stages in the gizzard and intestinal lumen. The effects of this outcome on broiler performance remainto be reported