After successful initiation of Phaius tankervilliae, we divided the initiated shoot in such a way that each piece should not be more than 1 cm and must contain two nodes. Then the pieces were cultured in sterilized ½, ¾ and standard MS media in the presence of 1 mg l−1 and 0.1 mg l−1, BAP (6-benzylaminopurine) and NAA (α-naphthalene acetic acid), respectively. These concentrations of BAP and NAA used here were same as used by Kevers and colleagues (Kevers et al. 2003). We observed more extensive multiplication in ¾ MS media as compare to other concentrations of MS media used.