Various settings on the homogenization equipment were studied such as the number
of passes, speed (rpm), duration (min), and pressure (psi). The traditional mixing method using a magnetic stirrer
was used as a control. The size of microcapsules resulting from the homogenization technique, and the various settings
were measured using a light microscope and a stage micrometer. The smallest capsules measuring (31.2 μm)
were created with the microfluidizer using 26 passes at 1200 psi for 40 min. The greatest loss in viability of 3.21
log CFU/mL was observed when using the ultra-turrax benchtop homogenizer with a speed of 1300 rpm for 5 min.
Overall, both homogenization techniques reduced capsule sizes; however, homogenization settings at high rpmalso
greatly reduced the viability of probiotic organisms