Results and Discussion
Of the various treatments assessed having individual
and combined BAP and NAA concentration, MS
medium supplemented with 1.0 µM BAP and 0.05 µM
NAA produced highest number of nodes, (7.45±0.6),
shoot length (63.30±5.3) and ö calli (10.20± 0.4)
(Table 1), (Fig. 1). About 60–65 mm shoot length and
6.65 nodes were observed on MS medium supplemented
with 0.25 µM BAP and 0.05 µM NAA (Fig. 2). With
increasing concentrations of BAP in the initiation of
calli were also found increasing in media supplemented
with BAP alone or combined BAP and NAA. MS
medium supplemented with 0.1, 0.25, 0.5 and 1.0 µM
BAP each with 0.5 µM NAA also initiated good
regeneration of nodes and increase in shoot length.
Among all the experiments, the best multiplication of
nodes and increased shoot length were recorded on
MS medium supplemented with 1.0 µM BAP and 0.5
µM NAA. In the same way high multiplication of nodes
and increased shoot length were recorded on MS
medium with 0.25 µM BAP and 0.5 µM NAA. Last
treatment of 1.0 µM NAA with factorial combinations
of BAP showed slightly less shoot numbers than in
former media.
medium supplemented with thidiazuron and kinetin (1.0
µM each).
Our results corroborate with the above results in
which low concentrations of BAP from 0.5 to 1.0 µM
favored best propagation of shoots and nodes. So the
present investigation suggests that the concentrations
1.0 µM BAP and 0.05 µM NAA are suitable for the
propagation of B. variegata plants.