Importance of OXYGEN Importance of CARBON DIOXIDE
• Normal ambient O2, concentrations are needed for normal embryonic development • Hypoxia can occur with low porosity shells and can cause embryonic mortality • Hypoxia slows growth rates • Naturally occurring hypoxia at end of incubation (blood and cell air)
• Naturally occurring metabolic acidosis with embryonic development :blood pCO2 compensated by increased HCO3- • Before external pipping : CO2 concentration can reach 8.5 % in air cell • Increasing tolerance of embryo to high ambient CO2 • IMPLICATION in SINGLE stage : decreasing aeration causes increase in CO2 during first half of incubation.