(1) Carrageenan-Induced Paw Edema. In this experiment, a
mark was made on both of the hind paws just below the
tibiotarsal junction so that every time the paw could be
dipped in the mercury column of plethysmograph up to the
mark to ensure constant paw volume. After 30 min of the
above treatment, an inflammatory edema was induced in the
left hind paw by injecting 0.1mL carrageenan (1%) in the
planter tissue hind paw of all the animals. The right paw
served as a reference to noninflamed paw for comparison.
The initial paw volume was measured plethysmographically
within 30 sec of the injection. The relative increase in the
paw volume was measured in control, standard, and treated
groups, 4 h after carrageenan injection. The percent increase
in paw volume over the initial reading was calculated. This
increase in the paw volume in animals treated with standard
drug and the different doses of methanol extract of the dried
fruits of C. carandas were compared with the increase in paw
volume of untreated control animals after 3 h.The percentage
inhibition of edema volume was calculated using the formula