The process was repeated until axenic cultures of different
microalgae were obtained. The microalgae were grown in separate
2 L flasks using the BG-11 medium (pH 7.4), incubated at temperature
30 C and 100 rpm for aeration. Artificial photoperiod of 18 h
light and 6 h dark was provided by using four compact fluorescent
lamps (50 Weach).