White-rot fungus Pleurotus floridanus LIPIMC996, obtained from
Laboratory of Microbial Systematic and LIPI Microbial Collection
(Lembaga Ilmu Pengetahuan, Cibinong, Indonesia) was used in this
work. The fungus was maintained on lignocellulosic medium at
room temperature before it was used as inoculums. Yeast strain
Saccharomyces cerevisiae CBS 8066 obtained from Centraalbureau
voor Schimelcultures (Delft, the Netherlands) was used for the
fermentation experiments. It was maintained on YPD agar plate
containing 20 g/L agar (Scharlau), 10 g/L yeast extract (Scharlau),
20 g/L peptone (Fluka), 20 g/L D-glucose (Scharlau) and stored at
4 C. Cellulase enzyme Cellic Ctec2 (Novozymes, Denmark) was
used for the hydrolysis. The enzyme had 168 FPU/mL activity,
determined according to the National Renewable Energy
Laboratory (NREL) method (Adney and Baker, 2008).
White-rot fungus Pleurotus floridanus LIPIMC996, obtained fromLaboratory of Microbial Systematic and LIPI Microbial Collection(Lembaga Ilmu Pengetahuan, Cibinong, Indonesia) was used in thiswork. The fungus was maintained on lignocellulosic medium atroom temperature before it was used as inoculums. Yeast strainSaccharomyces cerevisiae CBS 8066 obtained from Centraalbureauvoor Schimelcultures (Delft, the Netherlands) was used for thefermentation experiments. It was maintained on YPD agar platecontaining 20 g/L agar (Scharlau), 10 g/L yeast extract (Scharlau),20 g/L peptone (Fluka), 20 g/L D-glucose (Scharlau) and stored at4 C. Cellulase enzyme Cellic Ctec2 (Novozymes, Denmark) wasused for the hydrolysis. The enzyme had 168 FPU/mL activity,determined according to the National Renewable EnergyLaboratory (NREL) method (Adney and Baker, 2008).
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