Total phenolic content was determined in herbal infusions prior and after extraction with petroleum ether using Folin-Ciocalteu assay , with some modifications. Thus 100 uL of each tested infusion, 500 L of Folin-Ciocalteu reagent and 6 mL of deionized water were transferred in a 10 mL flask and mixed thoroughly. After 3 min, 1.5 mL of 20% Na2CO3 was added in the flask which was filled to 10 mL with water. After two hours standing, absorbance was measured at 725 nm. Caffeic acid was used as a standard (0–500 mg/L) and the results were expressed as mg caffeic acid per mL. All measurements were performed in triplicate.