2.2.1. Pre-treatment of the residues
DOR samples (50 g) were submitted to water extraction
(200 mL), to isolate soluble carbohydrates, at 40 and 100 C
(DOR40 and DOR100, respectively) and with 200 mL of aqueous
H2SO4 (1 mol dm3) at 100 C (DOR100H), during 2 h. DOR100H
was further neutralized with aqueous NaOH (1 mol dm3), and
final volume adjusted to 400 mL.