After sterilization,
each flask was inoculated with five discs of
mycelium and agar, 6 mm in diameter, agar plate
cultures of the fungus on complete medium (Raper
and Miles, 1958). Three replicates were prepared for
each treatment, and an uninoculated flask served as
control. All of the flasks were incubated at 25jC in
the dark. The entire contents of flasks were harvested
at 15, 20, 25, 30 days for the assay of a-amylase
activity. Then the samples were dried to constant
weight at 70jC and used for the assay of the main
nutritional components.