Data on the integrity of plasma and acrosomal membranes assessed by flow cytometry are shown in Fig. 2. The percentage of IPIA in the mMS extender was higher than that in PFM throughout the 6 h after thawing (P < 0.05).Higher percentages of spermatozoa with damaged acrosomal membranes (IPDA and DPDA) were observed in the PFM treatment group compared with the mMS group(P < 0.05). Although the percentage of sperm with damaged acrosomal membrane in the mMS group did not changed from 0.5 to 6 h after thawing, the percentage in PFM treat-ment was significantly (P < 0.05) higher at 3 and 6 h than at 0.5 h post-thawing. The percentages of DPIA at 3 and 6 h after thawing in the PFM group were higher than those in the mMS group (P < 0.05).