Addition of 5 mg GPE ml21 caused a similar increase in ROS and depletion of GSH, but a
decrease in NADH autofluorescence was also observed. A
similar response was exhibited by exponential-phase cells
(not shown). Low levels of ROS were localized in the
mitochondria in control cells (Fig. 5aii); increased CMDCF
fluorescence was observed in both mitochondrial
and cytoplasmic compartments after treatment with either
GPE or AA (Fig. 5bii and 5cii).