2.5. Scanning Electron Microscopy (SEM)
Dried and finely ground heat-moisture treated and
native waxy and non-waxy starches were mounted on
aluminum specimen stubs with double-sided adhesive
tape. Samples were sputter-coated in vacuo with a thin
film of gold–palladium in a Hummer II Versatile DC
Sputtering system (Technics, Alexandria, VA). Coated
samples were viewed in a Hitachi S-510 scanning electron microscope operating at an accelerating voltage
of 5 kV. Images were photographed at 3000 magnification on a Polaroid type 55 film.