Half of the infested fruit was randomly divided into five groups
for chemical sampling at different stages of infestation: egg, first
instar, second instar, mid-third instar, and exiting third instar (final
instar larvae exit the host fruit and enter a wandering stage prior to
pupation in the soil). Two control treatments were also sampled;
they consisted of non-infested fruit and mechanically injured fruit
that were pierced with a tack five times to simulate oviposition
wounds (tack length approximated length of A. suspensa ovipositor,
2.0 mm). The remaining half of the infested fruit was used tomonitor progress of larval development. At 2–3 d intervals, several
grapefruits were cut open to determine the larval instar and to
estimate the level of infestation. Each segment was opened and the
pulp separated and gently pressed to dislodge larvae. The albedo,
pulp and juice were then examined under a microscope to detect
larvae. Until the time of chemical sampling, all fruit treatments
were held in the laboratory at the same environmental conditions
used for insect rearing. Following chemical collections, each sampled
fruit was cut open and examined (as above) to document the
developmental stage and the number of insects present.