A cross-sectional study was conducted to determine microbial contamination of pig
carcasses at a slaughterhouse in Vientiane, capital of Lao People Democratic Republic (Lao
PDR). Between November 2004 and April 2005, 62 pig carcasses were randomly selected.
From each carcass, pooled swabs (from “1” prior to and “2” after evisceration) and 25 g of
tissue of mesenteric lymph nodes (MLN) were collected. The swab samples were examined for
Aerobic Plate Count (APC) and Enterobacteriaceae Counts (EBC) and cultured for Salmonella.
The lymph nodes were cultured for Salmonella only. Swabs1 and 2 had mean APC of 4.70 and
4.85 log10CFU/cm2, respectively. These two means were significantly (p=0.0001) different.
The means of EBC were 2.81 log10CFU/cm2 for Swab 1, and 2.98 log10CFU/cm2 for Swab 2.
The difference were also statistical significant (p=0.0001). The frequency of Salmonella isolation
from Swab 1 was 46.8%, for Swab 2 was 66.1%, and from mesenteric lymphnodes was
53.2%. Eight different Salmonella serotypes were identified. The most frequent (29.1%) serotype
was S. Rissen, followed by S. Anatum (26.2%), S. Derby (18.4%), and S. Elisabethville
(8.7%). The other serotypes identified were S. Amsterdam (7.8%), S. Typhimurium (4.9%), S.
Agona (2.9%), and S. Enteritidis (1.9%). Results of this study showed the levels of contamination
with aerobic bacteria and Enterobacteriaceae were higher than recommended standards,
and the carcasses were contaminated with Salmonella.
A cross-sectional study was conducted to determine microbial contamination of pig
carcasses at a slaughterhouse in Vientiane, capital of Lao People Democratic Republic (Lao
PDR). Between November 2004 and April 2005, 62 pig carcasses were randomly selected.
From each carcass, pooled swabs (from “1” prior to and “2” after evisceration) and 25 g of
tissue of mesenteric lymph nodes (MLN) were collected. The swab samples were examined for
Aerobic Plate Count (APC) and Enterobacteriaceae Counts (EBC) and cultured for Salmonella.
The lymph nodes were cultured for Salmonella only. Swabs1 and 2 had mean APC of 4.70 and
4.85 log10CFU/cm2, respectively. These two means were significantly (p=0.0001) different.
The means of EBC were 2.81 log10CFU/cm2 for Swab 1, and 2.98 log10CFU/cm2 for Swab 2.
The difference were also statistical significant (p=0.0001). The frequency of Salmonella isolation
from Swab 1 was 46.8%, for Swab 2 was 66.1%, and from mesenteric lymphnodes was
53.2%. Eight different Salmonella serotypes were identified. The most frequent (29.1%) serotype
was S. Rissen, followed by S. Anatum (26.2%), S. Derby (18.4%), and S. Elisabethville
(8.7%). The other serotypes identified were S. Amsterdam (7.8%), S. Typhimurium (4.9%), S.
Agona (2.9%), and S. Enteritidis (1.9%). Results of this study showed the levels of contamination
with aerobic bacteria and Enterobacteriaceae were higher than recommended standards,
and the carcasses were contaminated with Salmonella.
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A cross-sectional study was conducted to determine microbial contamination of pig
carcasses at a slaughterhouse in Vientiane, capital of Lao People Democratic Republic (Lao
PDR). Between November 2004 and April 2005, 62 pig carcasses were randomly selected.
From each carcass, pooled swabs (from “1” prior to and “2” after evisceration) and 25 g of
tissue of mesenteric lymph nodes (MLN) were collected. The swab samples were examined for
Aerobic Plate Count (APC) and Enterobacteriaceae Counts (EBC) and cultured for Salmonella.
The lymph nodes were cultured for Salmonella only. Swabs1 and 2 had mean APC of 4.70 and
4.85 log10CFU/cm2, respectively. These two means were significantly (p=0.0001) different.
The means of EBC were 2.81 log10CFU/cm2 for Swab 1, and 2.98 log10CFU/cm2 for Swab 2.
The difference were also statistical significant (p=0.0001). The frequency of Salmonella isolation
from Swab 1 was 46.8%, for Swab 2 was 66.1%, and from mesenteric lymphnodes was
53.2%. Eight different Salmonella serotypes were identified. The most frequent (29.1%) serotype
was S. Rissen, followed by S. Anatum (26.2%), S. Derby (18.4%), and S. Elisabethville
(8.7%). The other serotypes identified were S. Amsterdam (7.8%), S. Typhimurium (4.9%), S.
Agona (2.9%), and S. Enteritidis (1.9%). Results of this study showed the levels of contamination
with aerobic bacteria and Enterobacteriaceae were higher than recommended standards,
and the carcasses were contaminated with Salmonella.
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การศึกษาภาคตัดขวาง มีวัตถุประสงค์เพื่อศึกษาการปนเปื้อนของจุลินทรีย์ในซากสุกรในโรงฆ่าสัตว์
ในเวียงจันทน์ เมืองหลวงของสาธารณรัฐ ประชาธิปไตยประชาชนลาว ( สปป. ลาว
) ระหว่างเดือนพฤศจิกายน 2547 เมษายน 2005 , 62 หมูซากสุ่ม .
จากแต่ละซาก รวมจาก ( จาก " 1 " ก่อนและหลัง " 2 " evisceration ) และ 25 กรัม
แล้วเนื้อเยื่อของต่อมน้ำเหลือง ( มิน ) เก็บรวบรวม เช็ดล้างจำนวนตรวจสอบ
นับแผ่นแอโรบิก ( APC ) และผิดเพี้ยนนับ ( EBC ) และการเลี้ยงเชื้อ Salmonella .
ต่อมน้ำเหลืองคือ การเลี้ยงเชื้อเท่านั้น swabs1 2 เฉลี่ย APC ของกระแสเงินทุนและ
4.85 log10cfu / cm2 ) ทั้งสองวิธีการอย่างมีนัยสำคัญทางสถิติ ( P = 0.0001 ) ที่แตกต่างกัน .
หมายถึง EBC เป็น 281 log10cfu / cm2 สำหรับกวาด 1 , และ 2.98 log10cfu / cm2 สำหรับไม้กวาด 2 .
ความแตกต่างมีนัยสำคัญทางสถิติ ( p = 0.0001 ) ความถี่ของเชื้อซัลโมเนลลาที่แยกจากไม้กวาด
1 46.8 เปอร์เซ็นต์ สำหรับกวาด 2 66.1 % และจากแล้ว lymphnodes คือ
53.2 % แปด ( Salmonella ที่แตกต่างกันมีการระบุ . ที่พบบ่อยที่สุด ( 29.1 เปอร์เซ็นต์ ) หรือเป็น S
rissen , ตาม โดย anatum ( 26.2 % ) , S . Derby ( 184% ) , และ elisabethville
( 8.7% ) อื่น ๆ ( ระบุเป็นเอสอัมสเตอร์ดัม ( 7.8% ) , S . typhimurium ( 4.9% ) , S .
Agona ( 2.9% ) , และ enteritidis ( 1.9% ) ผลการศึกษาพบว่าระดับการปนเปื้อนของแบคทีเรียแอโรบิก และผิดเพี้ยน
สูงกว่ามาตรฐานแนะนำ
และซากถูกปนเปื้อนกับเชื้อ Salmonella .
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