The experiments used two treatments: (a)a control for bacterial effects: chlorine bleach (50 mg L−1 sodium hypochlorite), which is the standard treatment in The Netherlands,(b) and a mixture of 50 mM calcium chloride, 0.05 mM ancymidol(50 ml L−1Reducymol), sucrose 25 g L−1and a pH of 3.5 due to abuffer of 0.3 M citric acid and 0.2 M K2HPO4.