The extracted DNA was used as a template for
PCR amplification using primers ITS1 and ITS4,
which amplify a region of 840 bp across the genomic
region between the 18S and the 28S ribosomal
RNA genes that includes ITS1 (internal transcribed
spacer 1), 5.8S ribosomal DNA, and ITS2(int ernal
transcribed spacer 2). The ITS1 and ITS2 spacer regions
differ among species of yeast and have recently
been used to assess the phylogenetic relationship of
strains belonging to the genus Saccharomyces and to
assign strains to three closely related species of the genus: S. cerevisiae, S. bayanus (=S. uvarum), and
S. paradoxus (=S. douglasii) (Montrocher et al. 1998).