In an attempt to ensure successful fermentation of sour mustard green in the factory scale, two levels of LAB starter culture (obtained from BIOTEC culture collection) were used. There were three treatments in this study, one tank of control (natural fermentation), two tanks inoculated with starter culture at low-inoculum level, and two tanks inoculated with high-inoculum level. Approximately 4,500 kg of mustard green were fermented in each experimental tank. Brines and other ingredients were added to the vegetable according to the factory recipe. The fermentation process was conducted with minimum agitation at an ambient temperature for 12 days. The liquid suspension in the tanks were sampling for analysis periodically at day 0, 3, 7, and 12. Duplicate samples were collected from each tank, kept on ice and shipped to the laboratory for analysis in the next day.