The pH of
the medium was 5.0, and it had been sterilized at 110 C for
10 min prior to use. The flasks were incubated at 30 C and
150 rpm for 24 h to grow the inocula. After 24 h of incubation,
the medium was centrifuged (Hettich Zentrifugen Rotina 38R) at
10000g for 10 min and the solid cells were removed after centrifugation
to obtain the inoculum that would be used in the fermentation
assays.