2.4.4. Enzyme activity
Immediately after removal from the sealed pouch=2 g of tissue was flash frozen in liquid nitrogen and stored at -80c for later analysis when frozen tissue was ground to a powder with fine homogenous talc-like consistency using a SPEX SamplePrep cryomill (2 cycles each of 1 min pre-cool, 1 min run at 7 collisions per s and 1 min cool; Model 6870,Metuchen,NJ,USA).