The working FRAP reagent was prepared by mixing 10 volumes of 300 mmol/l acetate buffer, pH 3.6, with 1 volume of 10 mmol/l TPTZ (2,4,6-tripyridyl-s-triazine) in 40 mmol/l hydrochloric acid and with 1 volume of 20 mmol/l ferric chloride
Theworking FRAP reagent was prepared by mixing 10 volumes of300 mmol/l acetate buffer, pH 3.6, with 1 volume of 10 mmol/lTPTZ (2,4,6-tripyridyl-s-triazine) in 40 mmol/l hydrochloric acidand with 1 volume of 20 mmol/l ferric chloride