The determination of lipid peroxidation inhibi-tion activity (LPI) was adapted using the procedure described by Serratosa, Marquez, Lopez-Toledano, Medina, and Merida (2011).
The methanolic extract was prepared by extracting 1 g flour with methanol (methanol/water ratio of 80:20 (v/v)) and diluted to 50 mL.
Concentration for 50% of maximal effect (EC50) was obtained by interpolation from linear regression analysis and represented the effective concentration of methanolic extract (mg/mL) at which the DPPH radicals were scavenged by 50%, the absorbance was 0.5 for reducing power, and lipid peroxidation was inhibited by 50%, respectively.