After liquefaction, the slurry, now called “corn mash,”
is cooled to approximately 30°C (86°F), and a second
enzyme (glucoamylase) is added. Glucoamylase completes
the breakdown of the starch into simple sugar
(glucose). This step, called “saccharification,” often occurs
while the mash is filling the fermentor in preparation
for the next step (fermentation) and continues
throughout the next step.