The distillation process was initiated at constant reflux until the unit temperatures stabilized (stationary state), and then the studied reflux condition was set. At this point, 10 mL samples of the distillate current were removed consecutively, and composition analyses were carried out using a pycnometer to determine the density of the samples. This procedure was continued until the ethanol in this stream was exhausted. The best operational condition was used to evaluate the distillation of ethanol obtained from banana culture