and protected from light after preparation. Stock solutions
of the seed oils (1 mg/mL) were prepared and diluted to
final concentrations of 500, 250, 200, 100 and 50 毺g/mL in
methanol. 1 mL of 0.1 mM DPPH methanol solution was
added to solutions of the sample or standards (毩-tocopherol
and gallic acid separately) and incubated for 30 min in the
dark. The absorbance was determined at 518 nm. Blank
experiment was also carried out to determine the absorbance
of DPPH before interacting with the sample. The antioxidant
activity, AA was calculated using the equation given below.
The IC50 was determined on graphpad prism 3 software
through a non-regression analysis. The IC50 was taken as the
concentration that scavenged 50% of the radicals.