The aseptic culture of Nymphaea was started in the past two decades. Swindells [9] successfully developed tissue culture procedures for hardy waterlilies to eliminate fungal and bacterial contamination which was a major problem at that time. A few years later, Lakshmanan [10] cultured rhizome tips in vitro to multiply Nymphaea hybrid ‘James Brydon’ on Murashige and Skoog medium supplemented with different concentrations and combinations of plant growth regulators. Recently, Nymphaea cv. ‘Jongkolnee’ and ‘Joey Tomocik’ were multiply through bulbils and rhizome tips, respectively [11], [12]. Even if many achievements had been done in several varieties of Nymphaea, tissue culture of Thai native
varieties of day-blooming waterlilies, especially ‘Bua Phuean’ was still concealed. In order to prevent the loss of this native species, the in vitro culture of ‘Bua Phuean’ was investigated to
find the effect of plant growth regulator and activated charcoal on ‘Bua Phuean’ micropropagation.