The upper plate containing MEA was inoculated
on three spots with 10 ml of a c.a. 106 spores/ml suspension of
P. verrucosum Pv21. On the lower plate, 100 ml of a c.a. 107 cells/ml
suspension of D. hansenii were spread onto YMA. After drying, the
two plates were faced each other, sealed with parafilm and incubated
at 20 C during 14 days. Mouth-to-mouth plates without
D. hansenii inoculum on the lower plate were used as controls.
Trials were done in triplicate. Inhibition was determined by
measuring the mould colony diameter. The inhibitory activity was
expressed as previously described in Section