Flasks wereinoculated withaninitial 1.5±3.0g/lfresh orrecycledC.shehataeFPL-Y-049andshakenat100rpmat
25°C.Thewoodhydrolyzate wasinitially diluted20%
byadding nitrogenousnutrientsandcellinoculum. The
pHofacidhydrolyzatesofvariousbatcheswasadjusted
to 5.5±6using1Msodiumhydroxide.Theshake¯ask
fermentationwasoptimized withvariousreducedlevels
ofnitrogennutrients withfreshorrecycled cellsin the
presenceorabsenceof10mg/lzincsulfate