Alkaline treatment is the traditionally followed procedure for extraction of proteins from tomato seed meal and other complex plant matrices, such as industrial by-products. However, chemical methods could induce side-reactions, such as hydrolysis and extraction of non-protein components, and denaturation of protein with obvious effects on their functional properties. In order to overcome those drawbacks, enzymatic methods were developed , and preferred for their milder process conditions, easier control of reaction parameters and minimal secondary product formation . To our knowledge, very limited information is available regarding the use of enzymatic hydrolysis to extract protein from the byproducts of tomato processing industries, like DTSM, to develop new products.