A 5-g muscle
sample was homogenized in 25 mL of phosphate buffer (0.05 M,
pH 7) for 1 min at 12,000 rpm in an ice bath. The homogenate
was centrifuged at 7000g for 2 min at 4 C. The supernatant fraction
was filtered through glass wool and used to determine catalase,
GSH-Px and SOD activity. All the analysis was performed in
triplicate