2. In Vitro and In Vivo Toxicology Studies
2.1. Cytotoxicity. Varalakshmi et al. [21] evaluated the in
vitro antiproliferative effects of the aqueous extracts of
dried fruit rind of G. indica (0, 50, 100, 200 μg/mL)
on Balb/c 3T3 mouse fibroblasts and human peripheral
lymphocytes. The results showed that G. indica extracts
inhibited lymphocytes and 3T3 fibroblast cell survival. Thus,
the authors concluded that G. indica extracts exhibited
pronounced cytotoxic effects. However, there was a flaw
in their methodology, since the authors also reported that
Azadirachta indica and Coleus aromaticus exhibited cytotoxic
effects on lymphocytes despite the low cell viability in the
control group (only 50–55% of viable lymphocytes). In the
case of G. indica, percentage of viability in lymphocytes was
not even mentioned. Thus, definitive conclusion of G. indica
induced cytotoxicity could not be drawn due to the poorlydescribed methodology of their study