2.2.7. Polymorphism and X-ray diffraction
The polymorphic form of the fat crystals was determined via
X-ray diffraction according to the AOCS Cj 2-95 method (AOCS,
2009). The analyses were performed using a Philips (PW 1710)
diffractometer using the Bragg–Brentano geometry (H:2H) with
Cu-Ka radiation (k: 1.54056 Å, voltage: 40 kV, current: 30 mA).
Step sizes of 0.02 (2H scale) and an acquisition time of 2 s were
utilized in the scanning range 15–27 (2H scale). The samples
were melted at 70 C and maintained at 20 C for 24 h in an oven
for stabilization (Schenck & Peschar, 2004; Yap, deMan, & deMan,
1989).