A dye is a coloured substance that has an affinity to the substrate to which it is being applied. The majority of
natural dyes are from plant sources – roots, berries, bark, leaves, and wood, also from animals and microbes.
Natural dyes are non-toxic, non-polluting and less health hazardous. Moreover, their antioxidant and
antimicrobial nature further adds to their positive effects. The main idea of extracting dyes from natural sources
is to avoid the environmental pollution and also to avoid toxic and allergic reactions associated with synthetic
dyes. These natural dyes have emerged as an important alternative to synthetic dyes. In the present study, The
pure cultures of fungal strains, Trichodermaspand Aspergillussp were isolated from soil samples using PDA
plates. The fungal cultures were grown under static condition in PDB for the production of pigment. These
pigments were tested for their colour production properties by taking absorbance at different time intervals
during the incubation period. A set of 3 pieces of cloth (cotton, silk & silk cotton) were used for dyeing with the
fungal pigments in both pre mordanted and unmordanted conditions. The fungal filtrates were bio autographed
using chromatographic techniques (TLC & CC) to purify and identify the compounds present in it. The purified
samples was analyzed further by GC – MS and the compounds present in the filtrates were identified.
Keywords :Natural dyes, Aspergillussp. Trichodermasp. ,GC-MS