The latex was sampled from 32 Hevea brasiliensis trees from RRIM600 clone located on an area of the rubber plantation of Prince of Songkla University, Surat Thani Campus, Thailand. The trees were planted in 1990 and started tapping in 1997. The 32 selected rubber trees were tapped in the early morning. The following tapping system was used: S/2, 3d4 (the trees are tapped on one half spiral cut tapped downward 3 out of 4 days without stimulation). Latex was collected from the clean cup about 3 hours after tapping and filtered through a stainless steel sieve (pore size, 1.0 mm). This fresh latex was used for the cubic coagula and mini-cup coagula samples. The periods of sampling were from June and September 2012.