The present study demonstrated that the dissolution temperature has a significant impact on the physicochemical characteristics of the regenerated keratin-based materials. With dissolution temperature increased from 120 to 180 °C, the yield of keratin material reduced from 57% to 18% with respect to the mass of raw wool. This is attributed to generation of water soluble free amino acids and low molecular weight peptides. In particular, the cysteine content in keratin reduced drastically from an average of 10 mol% in raw wool to 1 mol% in regenerated keratin produced via dissolving wool at 180 °C followed by precipitation with water, similar to the other keratin materials processed at high temperature environment such as steam explosion or super-heated water treatment. This low level of cysteine in regenerated keratin obtained at high temperature is advantageous with respect to thermal processing of keratin as demonstrated in compression moulding and DSC analyses, and also reflected in their microstructural morphology. As cysteine acts as a source or catalyst in creating disulphide cross-linking among keratin chains, without this amino acid keratin chains are mobile and are effectively plasticized with glycerol. SEM images indicated that the cross-sectional surfaces of sheets of regenerated keratins contained dense fibrous network, which was more compact when high dissolution temperature (180 °C) was used. Keratin material regenerated at high temperature formed low modulus and stretchable cohesive films as demonstrated by stress-strain characteristics. In summary, although further development is required to create keratinous materials that are melt-processable like classical synthetic thermoplastics, this study has determined that keratin regenerated from ionic liquid dissolution of wool followed by coagulation with water, produces a proteineous derivative suitable for industrial applications.